本研究成功开发了一种用于检测牛朊病毒蛋白基因（PRNP）中12 bp和23 bp插入缺失多态性（indel）的四引物扩增阻滞突变系统PCR（tetra-ARMS PCR）方法。该方法克服了传统测序和PCR-RFLP等技术的繁琐、耗时和高成本等局限，仅需常规PCR仪和电泳设备即可实现准确、快速 ...

Due to spontaneous deficiency in leptin, ob/ob mice are one of the most commonly used experimental animal models in diabetes research. In this study, we reported a quick and easy-to-conduct genotyping ...