Affinity chromatography has emerged as an indispensable tool in modern protein purification, leveraging highly selective interactions between target proteins and immobilised ligands. This technique, ...

Three-phase partitioning (TPP) is an efficient and cost-effective method for protein purification that exploits the simultaneous addition of salts and organic solvents to an aqueous protein extract.

The purification of proteins and antibodies is a critical process in biotechnology and pharmaceutical industries. Traditional methods often involve single-use materials, leading to high costs and ...

Vortex fluidic devices (VFDs) used with immobilized metal affinity chromatography (IMAC) have provided an effective, albeit cumbersome, way to separate His-tagged proteins. Researchers recently ...

Researchers at Queen’s University in Kingston, Ontario, Canada, are developing an alternative to nickel-nitrilotriacetic acid (Ni-NTA) to purify recombinant proteins. Because it uses common ...