qPCR技术具有高度敏感性，实验的各个环节，无论是实验设备的精准性、耗材和试剂的稳定性、人员和环境的影响等因素均会造成从样品制备到结果分析的偏差。因此，在实验室环境、人员的操作等方面需严格控制，以避免交叉污染并保证qPCR检测结果的准确性和重复性。基于此，应对qPCR实验室开展能力验证，如发生检测结果与指定值或其他能力评价标准之间存在明显差异的情况，应及时调查潜在的误差或不满意结果的来源，识别存在 ...

Real-time quantitative PCR (RT-qPCR or qPCR) is revolutionizing many aspects of molecular biology and becoming the gold standard for accurate, sensitive, and fast quantification of gene expression.

Quantitative PCR (qPCR) is one of the most widely used tools in genomics. It is so powerful and user-friendly that researchers tend to over rely on it. Every technique has its limitations, and qPCR is ...

This 5-day training is designed to provide a solid understanding of specific topics through presentation and laboratory work. Participants will gain significant experience in the performance of ...

The gold standard for gene-expression analysis, qPCR, has been extensively studied. Standard protocols have been published and multiple instruments for cycling with real-time detection are ...

Explore digital PCR’s potential, how the technology has transformed life science research, the challenges it faces and what ...

Explore 40 years of PCR history, from Kary Mullis’s invention story to the rise of digital PCR and how it revolutionized ...